MOSAIC: A Modular Single-Molecule Analysis InterfaceΒΆ

MOSAIC is a single molecule analysis toolbox that automatically decodes multi-state nanopore data. By modeling the nanopore system with an equivalent circuit, MOSAIC leverages the transient response of a molecule entering the channel to quantify pore-molecule interactions. In contrast to existing techniques such as ionic current thresholding [Pedone:2009ds][Robertson:2010gm] or Viterbi decoding [Viterbi:1967hq], this technique allows the estimation of short-lived transient events that are otherwise not analyzed.

Nanometer-scale pores have demonstrated potential use in biotechnology applications, including DNA sequencing [Kasianowicz:1996us], single-molecule force spectroscopy [VanDorp:2009tg], and single-molecule mass spectrometry [Robertson:2007jo]. The data modeling and analysis methods implemented in MOSAIC allow for considerable improvements in the quantification of molecular interactions with the channel in each of these applications.