MOSAIC: A Modular Single-Molecule Analysis InterfaceΒΆ
MOSAIC is a single molecule analysis toolbox that automatically decodes multi-state nanopore data. By modeling the nanopore system with an equivalent circuit, MOSAIC leverages the transient response of a molecule entering the channel to quantify pore-molecule interactions. In contrast to existing techniques such as ionic current thresholding [Pedone:2009ds][Robertson:2010gm] or Viterbi decoding [Viterbi:1967hq], this technique allows the estimation of short-lived transient events that are otherwise not analyzed.
Nanometer-scale pores have demonstrated potential use in biotechnology applications, including DNA sequencing [Kasianowicz:1996us], single-molecule force spectroscopy [VanDorp:2009tg], and single-molecule mass spectrometry [Robertson:2007jo]. The data modeling and analysis methods implemented in MOSAIC allow for considerable improvements in the quantification of molecular interactions with the channel in each of these applications.
- 1. Introduction
- 2. Data Processing Algorithms in MOSAIC
- 3. Getting Started
- 4. MOSAIC GUI
- 5. MOSAIC WEB
- 6. Settings File
- 7. Database Structure and Query Syntax
- 8. Scripting and Advanced Features
- 9. Extend MOSAIC
- 10. Publication Quality Figures
- 11. Advanced Analysis
- 12. Addons
- 13. Developer Tools
- 14. API Documentation
- 15. Bibliography